Cloning of the genomic locus on chromosome 13 of a new human homeobox gene with strong homology to the rat somatostatin transactivating factor-1

Journal Article · Thu Sep 01 04:00:00 EDT 1994 · American Journal of Human Genetics

OSTI ID:134639

Russo, J; Venkatraj, V S; Tan, D F ^[1]

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1. Columbia Univ., NY (United States); and others

Degenerate PCR primers designed to amplify highly conserved homeobox motifs were used to amplify a chromosome 13-specific genomic phage library. A resulting PCR product was sequenced and found to have high homology to the Xenopus gene, Xhox 8. This PCR fragment was used to screen a chromosome 13-specific genomic cosmid library. Several cosmids were recovered and homeobox containing subclones were sequenced. The homeobox contained in these chromosome 13 cosmids was found by blast analysis to have near-perfect homology at the amino acid level to the rat somatostatin transactivating factor-1. The homeobox-containing cosmid clones were used as FISH probes and were found to map to the 13q12 region. Several cDNA libraries were screened with the homeobox-containing genomic clones, however, no positive colonies were recovered.

• OSTI ID: 134639
• Report Number(s): CONF-941009--
• Journal Information: American Journal of Human Genetics, Journal Name: American Journal of Human Genetics Journal Issue: Suppl.3 Vol. 55; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AMINO ACID SEQUENCE
COMPARATIVE EVALUATIONS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
FLUORESCENCE
GENE MUTATIONS
GENES
GENETIC MAPPING
GENETICS
HUMAN CHROMOSOME 13
HUMAN POPULATIONS
POLYMERASE CHAIN REACTION
RATS
SOMATOSTATIN