In situ Studies of Soft- and Reactive Landing of Mass-Selected Ions Using Infrared Reflection Absorption Spectroscopy

Journal Article · Tue Sep 01 04:00:00 EDT 2009 · Analytical Chemistry, 81(17):7302-7308

DOI:https://doi.org/10.1021/ac901149s· OSTI ID:965547

Hu, Qichi; Wang, Peng; Gassman, Paul L; Laskin, Julia

Grazing incidence infrared reflection absorption spectroscopy (IRRAS) for in situ and in real time characterization of substrates modified by soft- and reactive landing (SL and RL) of complex ions was implemented on a mass-selected ion deposition instrument. Ions produced by electrospray ionization were mass-selected using a quadrupole mass filter and deposited onto inert and reactive self-assembled monolayer (SAM) surfaces. Surface composition during and after ion deposition was monitored using IRRAS. Physisorption of a cyclic peptide, Garmicidin S (GS), was studied for 8 hrs during deposition and additional 12 hrs after the end of deposition. The integrated signal of the characteristic amide bands followed a linear increase during the deposition and stayed unchanged after the deposition was finished. Similar linear increase in IRRAS signal was obtained following reactive deposition of the protonated dodecanediamine onto SAMs of dithiobis (succinimidyl undecanoate) (NHS-SAM) and 16-mercaptohexadecanoic acid fluoride (COF-SAM) on gold. IRRAS allowed us to monitor for the first time the formation of the amide bond between reactive SAM surfaces and the projectile molecule.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 965547
• Report Number(s): PNNL-SA-66638; 19594; 30466; 30445; 34913; KC0302020
• Journal Information: Analytical Chemistry, 81(17):7302-7308, Journal Name: Analytical Chemistry, 81(17):7302-7308 Journal Issue: 17 Vol. 81
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
ABSORPTION SPECTROSCOPY
AMIDES
AMINES
COF-SAM
DEPOSITION
Environmental Molecular Sciences Laboratory
FLUORIDES
FSAM.
GOLD
Gramicidin S
Grazing Incidence Infrared Reflection-Absorption Spectroscopy (IRRAS)
IONIZATION
Ion soft-landing
NHS-SAM
PEPTIDES
SORPTION
SUBSTRATES
THIOLS
dodecanediamine
reactive landing
self-assembled monolayers (SAMs)
surface collisions