Contribution of mobile genetic elements to Desulfovibrio vulgaris genome plasticity

Journal Article · Thu Jan 01 04:00:00 EST 2009 · Environmental Microbiology

DOI:https://doi.org/10.1111/j.1462-2920.2009.01946.x· OSTI ID:963705

Walker, Christopher ^[1]; Stolyar, Sergey ^[2]; Chivian, Dylan ^[3]; Pinel, Nicolas ^[1]; Gabster, Jeffrey ^[1]; Dehal, Paramvir ^[3]; He, Zhili ^[4]; Yang, Zamin Koo ^[5]; Yen, Huei-Che ^[6]; Zhou, Jizhong ^[4]; Hazen, Terry ^[3]; Arkin, Adam ^[3]; Stahl, David ^[2]

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1. University of Washington, Seattle
2. University of Washington
3. Lawrence Berkeley National Laboratory (LBNL)
4. University of Oklahoma
5. ORNL
6. University of Missouri, Columbia

The genome of Desulfovibrio vulgaris strain DePue, a sulfate-reducing Deltaproteobacterium isolated from heavy metal-impacted lake sediment, was completely sequenced and compared with the type strain D. vulgaris Hildenborough. The two genomes share a high degree of relatedness and synteny, but harbour distinct prophage and signatures of past phage encounters. In addition to a highly variable phage contribution, the genome of strain DePue contains a cluster of open-reading frames not found in strain Hildenborough coding for the production and export of a capsule exopolysaccharide, possibly of relevance to heavy metal resistance. Comparative whole-genome microarray analysis on four additional D. vulgaris strains established greater interstrain variation within regions associated with phage insertion and exopolysaccharide biosynthesis.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 963705
• Journal Information: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 9 Vol. 11; ISSN 1462-2912
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOSYNTHESIS
DESULFOVIBRIO
EXPORTS
GENETICS
HEAVY METALS
LAKES
PLASTICITY
PRODUCTION
STRAINS