Recovery of temperate Desulfovibrio vulgaris bacteriophage on anovel host strain
Journal Article
·
· Environmental Microbiology
OSTI ID:903368
A novel sulfate-reducing bacterium (strain DePue) closelyrelated to Desulfovibrio vulgaris ssp. vulgaris strain Hildenborough wasisolated from the sediment of a heavy-metal impacted lake usingestablished techniques. Although few physiological differences betweenstrains DePue and Hildenborough were observed, pulsed-field gelelectrophoresis (PFGE) revealed a significant genome reduction in strainDePue. Comparative whole-genome microarray and PCR analyses demonstratedthat the absence of genes annotated in the Hildenborough genome as phageor phage-related contributed to the significant genome reduction instrain DePue. Two morphotypically distinct temperate bacteriophage fromstrain Hildenborough were recovered using strain DePue as a host forplaque isolation.
- Research Organization:
- COLLABORATION - U. ofWashington
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 903368
- Report Number(s):
- LBNL--60457; BnR: KP1102010
- Journal Information:
- Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 10 Vol. 8; ISSN 1462-2912
- Country of Publication:
- United States
- Language:
- English
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