Effect of Growth Temperature and Culture Medium on the Cryotolerance of Permafrost Exiguobacterium Sibiricum 255-15 by Proteome-Wide Mass Mapping

Journal Article · Thu Jan 01 04:00:00 EST 2009 · Open Proteomics Journal, The

DOI:https://doi.org/10.2174/1875039700902010008· OSTI ID:963415

Qiu, Yinghua ^[1]; Vishnivetskaya, Tatiana A ^[2]; Qiu, Weilian ^[1]; Lubman, David M ^[1]

---

1. University of Michigan
2. ORNL

Exiguobacterium sibiricum 255-15 has shown significantly improved cryotolerance after liquid broth growth at 4oC and agar surface growth at both 4oC and 25oC compared with liquid broth growth at 25oC. The ability to survive freeze-thaw stress is expected to depend on the physiological state and protein composition of cells prior to freezing. Using 2-D liquid separation and an ESI-TOF MS-based mass mapping technique, we examined the differences in the proteomic profiles of the permafrost bacterium E. sibiricum 255-15 grown at two temperatures (4oC and 25oC) and two media (liquid broth and agar surface) before freeze-thawing treatments. In this study, a total of 330 proteins were identified. The cells cultured under the growth conditions associated with the improved cryotolerance have revealed a general downregulation of enzymes involved in major metabolic processes (glycolysis, anaerobic respiration, ATP synthesis, fermentation, electron transport, and sugar metabolism) as well as in the metabolism of lipids, amino acids, nucleotides and nucleic acids. In addition, eight proteins (2 -5 RNA ligase, hypoxanthine phosphoribosyl transferase, FeS assembly ATPase SufC, thioredoxin reductase and four hypothetical proteins) were observed to be up-regulated. This suggests these eight proteins might have a potential role to induce the improved cryotolerance.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 963415
• Journal Information: Open Proteomics Journal, The, Journal Name: Open Proteomics Journal, The Vol. 2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
AMINO ACIDS
ELECTRONS
ENZYMES
FERMENTATION
FREEZING
GLYCOLYSIS
HYPOXANTHINE
LIPIDS
METABOLISM
NUCLEIC ACIDS
NUCLEOTIDES
OXIDOREDUCTASES
PERMAFROST
PROTEINS
RESPIRATION
RNA
SACCHAROSE
SYNTHESIS