Effect of low temperature and culture media on the growth and freeze-thawing tolerance of Exiguobacterium strains

Journal Article · Mon Jan 01 04:00:00 EST 2007 · Cryobiology

DOI:https://doi.org/10.1016/j.cryobiol.2007.01.008· OSTI ID:963402

Vishnivetskaya, Tatiana A ^[1]; Siletzky, Robin ^[2]; Jefferies, Natalie ^[2]; Tiedje, James M. ^[3]; Kathariou, Sophia ^[2]

---

1. ORNL
2. North Carolina State University
3. Michigan State University, East Lansing

Bacteria of the genus Exiguobacterium have been repeatedly isolated from ancient permafrost sediments of the Kolyma lowland of Northeast Eurasia. Here we report that the Siberian permafrost isolates Exiguobacterium sibiricum 255-15, E. sibiricum 7-3, Exiguobacterium undae 190-11 and E. sp. 5138, as well as Exiguobacterium antarcticum DSM 14480, isolated from a microbial mat sample of Lake Fryxell (McMurdo Dry Valleys, Antarctica), were able to grow at temperatures ranging from 6 to 40 C. In comparison to cells grown at 24 C, the cold-grown cells of these strains tended to be longer and wider. We also investigated the effect of growth conditions (broth or surface growth, and temperature) on cryotolerance of the Exiguobacterium strains. Bacteria grown in broth at 4 C showed markedly greater survival following freeze-thawing treatments (20 repeated cycles) than bacteria grown in broth at 24 C. Surprisingly, significant protection to repeated freeze-thawing was also observed when bacteria were grown on agar at either 4 or 24 C.

• Research Organization: Oak Ridge National Laboratory (ORNL)
• Sponsoring Organization: SC USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 963402
• Journal Information: Cryobiology, Journal Name: Cryobiology Vol. 54; ISSN CRYBAS; ISSN 0011-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ANTARCTICA
BACTERIA
CULTURE MEDIA
Cryotolerance
Exiguobacterium
FREEZING
Freeze-thawing
Freezing tolerance
GROWTH
Low temperature growth
PERMAFROST
Permafrost
TEMPERATURE DEPENDENCE
THAWING
TOLERANCE