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Title: Nucleotide sequences specific to Brucella and methods for the detection of Brucella

Patent ·
OSTI ID:961574

Nucleotide sequences specific to Brucella that serves as a marker or signature for identification of this bacterium were identified. In addition, forward and reverse primers and hybridization probes derived from these nucleotide sequences that are used in nucleotide detection methods to detect the presence of the bacterium are disclosed.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
W-7405-ENG-48; AC52-07NA27344
Assignee:
Lawrence Livermore National Security, LLC (Livermore, CA)
Patent Number(s):
7,494,773
Application Number:
10/630,607
OSTI ID:
961574
Country of Publication:
United States
Language:
English

References (5)

Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification journal September 1996
Specificity of a Polymerase Chain Reaction Assay of a Target Sequence on the 31-Kilodalton Brucella Antigen DNA Used to Diagnose Human Brucellosis
  • Casañas, M. C.; Queipo-Ortuño, M. I.; Rodriguez-Torres, A.
  • European Journal of Clinical Microbiology and Infectious Diseases, Vol. 20, Issue 2 https://doi.org/10.1007/PL00011242
journal March 2001
Direct detection of Brucella spp. in raw milk by PCR and reverse hybridization with 16S-23S rRNA spacer probes. journal January 1996
Serum Is the Preferred Clinical Specimen for Diagnosis of Human Brucellosis by PCR journal April 2001
Quantitative measurement of Stachybotrys chartarum conidia using real time detection of PCR products with the TaqManTMfluorogenic probe system journal October 1999