Light Flux Density Threshold at Which Protein Denaturation is Induced by Synchrotron Radiation Circula Dichroism Beamlines
New high-flux synchrotron radiation circular dichroism (SRCD) beamlines are providing important information for structural biology, but can potentially cause denaturation of the protein samples under investigation. This effect has been studied at the new CD1 dedicated SRCD beamline at ISA in Denmark, where radiation-induced thermal damage effects were observed, depending not only on the radiation flux but also on the focal spot size of the light. Comparisons with similar studies at other SRCD facilities worldwide has lead to the estimation of a flux density threshold under which SRCD beamlines should be operated when samples are to be exposed to low-wavelength vacuum ultraviolet radiation for extended periods of time.
- Research Organization:
- Brookhaven National Laboratory (BNL) National Synchrotron Light Source
- Sponsoring Organization:
- Doe - Office Of Science
- DOE Contract Number:
- AC02-98CH10886
- OSTI ID:
- 959753
- Report Number(s):
- BNL--82739-2009-JA
- Journal Information:
- Journal of Synchrotron Radiation, Journal Name: Journal of Synchrotron Radiation Vol. 15; ISSN 0909-0495; ISSN JSYRES
- Country of Publication:
- United States
- Language:
- English
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