Synchrotron Radiation Circular Dichroism (SRCD) Spectroscopy: An Emerging Method in Structural Biology for Examining Protein Conformations and Protein Interactions

Journal Article · Thu Sep 01 00:00:00 EDT 2011 · Nuclear Instruments and Methods in Physics Research, Section A

DOI:https://doi.org/10.1016/j.nima.2010.10.135· OSTI ID:1040563

Wallace, B A; Sutherland, J; Gekko, K; Hoffmann, S V; Lin, Y -H; Tao, Y; Wien, F; Janes, R W

Circular dichroism (CD) spectroscopy is a well-established technique in structural biology. The use of synchrotron radiation as an intense light source for these measurements extends the applications possible using lab-based instruments. In recent years, there has been a major growth in synchrotron radiation circular dichroism (SRCD) beamlines worldwide, including ones at the NSLS, ISA, SRS, HiSOR, BSRF, NSRRC, SOLEIL, Diamond, TERAS, BESSYII, and ANKA synchrotrons. Through the coordinated efforts of beamline scientists and users at these sites, important proof-of-principle studies have been done enabling the method to be developed for novel and productive studies on biological systems. This paper describes the characteristics of SRCD beamlines and some of the new types of applications that have been undertaken using these beamlines.

• Research Organization: BROOKHAVEN NATIONAL LABORATORY (BNL)
• Sponsoring Organization: USDOE SC OFFICE OF SCIENCE (SC)
• DOE Contract Number: AC02-98CH10886
• OSTI ID: 1040563
• Report Number(s): BNL--94000-2011-JA; KP1501010
• Journal Information: Nuclear Instruments and Methods in Physics Research, Section A, Journal Name: Nuclear Instruments and Methods in Physics Research, Section A Journal Issue: 1 Vol. 649; ISSN NIMAER; ISSN 0168-9002
• Country of Publication: United States
• Language: English

Related Subjects

43 PARTICLE ACCELERATORS
59 BASIC BIOLOGICAL SCIENCES
BEAM OPTICS
BIOLOGY
CONFORMATIONAL CHANGES
Conformational changes
DICHROISM
LIGHT SOURCES
Macromolecular interactions
PROTEIN STRUCTURE
PROTEINS
Protein secondary structure
SPECTROSCOPY
SYNCHROTRON RADIATION
Synchrotron radiation circular dichroism spectroscopy