Effect of Divalent Cations on the Structure of the Antibiotic Daptomycin

Journal Article · Tue Oct 30 00:00:00 EDT 2007 · European Biophysics Journal:EBJ, 37(4):421-433

DOI:https://doi.org/10.1007/s00249-007-0227-2· OSTI ID:959215

Ho, Steven W; Jung, David; Calhoun, Jennifer R; Lear, James D; Okon, Mark; Scott, Walter R; Hancock, Robert; Straus, Suzana K

Abstract Daptomycin, a cyclic anionic lipopeptide antibiotic, whose three-dimensional structure was recently solved using solution state NMR (Ball et al. 2004; Jung et al. 2004; Rotondi and Gierasch 2005), requires calcium for function. To date, the exact nature of the interaction between divalent cations, such as Ca2+ or Mg2+, has not been fully characterized. It has, however, been suggested that addition of Ca2+ to daptomycin in a 1:1 molar ratio induces aggregation. Moreover, it has been suggested that certain residues, e.g. Asp3 and Asp7, which are essential for activity (Grunewald et al. 2004; Kopp et al. 2006), may also be important for Ca2+ binding (Jung et al. 2004).

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 959215
• Journal Information: European Biophysics Journal:EBJ, 37(4):421-433, Journal Name: European Biophysics Journal:EBJ, 37(4):421-433 Journal Issue: 4 Vol. 37; ISSN EBJOE8; ISSN 0175-7571
• Country of Publication: United States
• Language: English

Related Subjects

60 APPLIED LIFE SCIENCES
ANTIBIOTICS
CALCIUM
CATIONS
Environmental Molecular Sciences Laboratory
RESIDUES