A variable DNA recognition site organization establishes the LiaR-mediated cell envelope stress response of enterococci to daptomycin

Davlieva, Milya; Shi, Yiwen; Leonard, Paul G.; Johnson, Troy A.; Zianni, Michael R.; Arias, Cesar A.; Ladbury, John E.; Shamoo, Yousif

doi:10.1093/nar/gkv321

A variable DNA recognition site organization establishes the LiaR-mediated cell envelope stress response of enterococci to daptomycin

Journal Article · Sun Apr 19 00:00:00 EDT 2015 · Nucleic Acids Research

DOI:https://doi.org/10.1093/nar/gkv321· OSTI ID:1223722

Davlieva, Milya ^[1]; Shi, Yiwen ^[1]; Leonard, Paul G. ^[2]; Johnson, Troy A. ^[2]; Zianni, Michael R. ^[3]; Arias, Cesar A. ^[4]; Ladbury, John E. ^[2]; Shamoo, Yousif ^[1]

Rice Univ., Houston, TX (United States)
The Univ. of Texas MD Anderson Cancer Center, Houston, TX (United States)
The Ohio State Univ., Columbus, OH (United States)
Univ. of Texas Medical School at Houston, Houston, TX (United States); The Univ. of Texas Health Science Center at Houston, Houston, TX (United States); Univ. El Bosque, Bogota (Columbia)

LiaR is a ‘master regulator’ of the cell envelope stress response in enterococci and many other Gram-positive organisms. Mutations to liaR can lead to antibiotic resistance to a variety of antibiotics including the cyclic lipopeptide daptomycin. LiaR is phosphorylated in response to membrane stress to regulate downstream target operons. Using DNA footprinting of the regions upstream of the liaXYZ and liaFSR operons we show that LiaR binds an extended stretch of DNA that extends beyond the proposed canonical consensus sequence suggesting a more complex level of regulatory control of target operons. We go on to determine the biochemical and structural basis for increased resistance to daptomycin by the adaptive mutation to LiaR (D191N) first identified from the pathogen Enterococcus faecalis S613. LiaR^D191N increases oligomerization of LiaR to form a constitutively activated tetramer that has high affinity for DNA even in the absence of phosphorylation leading to increased resistance. The crystal structures of the LiaR DNA binding domain complexed to the putative consensus sequence as well as an adjoining secondary sequence show that upon binding, LiaR induces DNA bending that is consistent with increased recruitment of RNA polymerase to the transcription start site and upregulation of target operons.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1223722

Alternate ID(s):: OSTI ID: 1229907

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: 9 Vol. 43; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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