Formation and dynamics of supported phospholipid membranes on a periodic nanotextured substrate
- Los Alamos National Laboratory
We have studied and modeled the morphology and dynamics of planar lipid bilayer assemblies supported on a textured silicon substrate that contains nano-structured features perpendicular to the plane of observation. Using a combination of characterization methods, including atomic force microscopy and quantitative fluorescence microscopy, we have discovered the bilayer assemblies are conformal to the underlying nanostructured substrate. As far as the dynamics are concnerned, the lipids freely diffuse in and out of the plane of observation while supported by the nanotextured material. In a microscopic sense, diffusion is isotropic along the patterned substrate. Macroscopically, when observed over length scales exceeding the dimensions of the nano-structured features, the diffusion appears anisotropic. The observed macroscopic anisotropy is well simulated using models of diffusion on the nanostructured surface that assume the lipids diffuse homogeneously and isotropically on the supporting nanotextured substrate.
- Research Organization:
- Los Alamos National Laboratory (LANL)
- Sponsoring Organization:
- DOE
- DOE Contract Number:
- AC52-06NA25396
- OSTI ID:
- 957717
- Report Number(s):
- LA-UR-08-04295; LA-UR-08-4295
- Journal Information:
- Langmuir, Journal Name: Langmuir; ISSN LANGD5; ISSN 0743-7463
- Country of Publication:
- United States
- Language:
- English
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