Carbon nanotube porin diffusion in mixed composition supported lipid bilayers

Sullivan, Kylee; Zhang, Yuliang; Lopez, Joseph; Lowe, Mary; Noy, Aleksandr

doi:10.1038/s41598-020-68059-2

Carbon nanotube porin diffusion in mixed composition supported lipid bilayers

Journal Article · Fri Jul 17 00:00:00 EDT 2020 · Scientific Reports

DOI:https://doi.org/10.1038/s41598-020-68059-2· OSTI ID:1638906

Sullivan, Kylee; Zhang, Yuliang; Lopez, Joseph; Lowe, Mary; Noy, Aleksandr

Carbon nanotube porins (CNTPs), short pieces of carbon nanotubes capable of self-inserting into a lipid bilayer, represent a simplified model of biological membrane channels. We have used high-speed atomic force microscopy (HS-AFM) and all-atom molecular dynamics (MD) simulations to study the behavior of CNTPs in a mixed lipid membrane consisting of DOPC lipid with a variable percentage of DMPC lipid added to it. HS-AFM data reveal that the CNTPs undergo diffusive motion in the bilayer plane. Motion trajectories extracted from the HS-AFM movies indicate that CNTPs exhibit diffusion coefficient values broadly similar to values reported for membrane proteins in supported lipid bilayers. The data also indicate that increasing the percentage of DMPC leads to a marked slowing of CNTP diffusion. MD simulations reveal a CNTP-lipid assembly that diffuses in the membrane and show trends that are consistent with the experimental observations.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States). The Molecular Foundry (TMF); Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDOE Office of Science (SC), Basic Energy Sciences (BES). Materials Sciences & Engineering Division

Grant/Contract Number:: AC02-05CH11231; AC52-07NA27344

OSTI ID:: 1638906

Alternate ID(s):: OSTI ID: 1642362

Report Number(s):: LLNL-JRNL--800281; 11908; PII: 68059

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Journal Issue: 1 Vol. 10; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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