High-Throughput Analysis of Serum Antigens Using Sandwich ELISAs on Microarrays
Book
·
OSTI ID:957363
Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection and validation of disease biomarkers. ELISA microarrays are capable of simultaneous detection of many proteins using a small sample volume. Although there are many potential pitfalls to the use of ELISA microarrays, these can be avoided by careful planning of experiments. In this chapter we describe a high-throughput protocol for processing ELISA microarrays that will result in reliable and reproducible data.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 957363
- Report Number(s):
- PNNL-SA-55713; 400412000; TRN: US201007%%670
- Resource Relation:
- Related Information: Tumor Biomarker Discovery, Methods in Molecular Biology, 520:143-150
- Country of Publication:
- United States
- Language:
- English
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