Sandwich ELISA Microarrays: Generating Reliable and Reproducible Assays for High-Throughput Screens
Book
·
OSTI ID:957360
The sandwich ELISA microarray is a powerful screening tool in biomarker discovery and validation due to its ability to simultaneously probe for multiple proteins in a miniaturized assay. The technical challenges of generating and processing the arrays are numerous. However, careful attention to possible pitfalls in the development of your antibody microarray assay can overcome these challenges. In this chapter, we describe in detail the steps that are involved in generating a reliable and reproducible sandwich ELISA microarray assay.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 957360
- Report Number(s):
- PNNL-SA-53301; 400412000; TRN: US201007%%667
- Resource Relation:
- Related Information: Biomarker Methods in Drug Discovery and Development, 273-290
- Country of Publication:
- United States
- Language:
- English
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