New Paradigm for Macromolecular Crystallography Experiments at SSRL: Automated Crystal Screening And Remote Data Collection

Journal Article · Tue May 26 04:00:00 EDT 2009 · Acta Crystallogr.D Biol.Crystallogr.D64:1210,2008

OSTI ID:953572

Soltis, S M; Cohen, A E; Deacon, A; Eriksson, T; Gonzalez, A; McPhillips, S; Chui, H; Dunten, P; Hollenbeck, M; Mathews, I; Miller, M; Moorhead, P; Phizackerley, R P; Smith, C; Song, J; Bedem, H van dem; Ellis, P; Kuhn, P; McPhillips, T; Sauter, N ; Sharp, K

Complete automation of the macromolecular crystallography experiment has been achieved at Stanford Synchrotron Radiation Lightsource (SSRL) through the combination of robust mechanized experimental hardware and a flexible control system with an intuitive user interface. These highly reliable systems have enabled crystallography experiments to be carried out from the researchers' home institutions and other remote locations while retaining complete control over even the most challenging systems. A breakthrough component of the system, the Stanford Auto-Mounter (SAM), has enabled the efficient mounting of cryocooled samples without human intervention. Taking advantage of this automation, researchers have successfully screened more than 200 000 samples to select the crystals with the best diffraction quality for data collection as well as to determine optimal crystallization and cryocooling conditions. These systems, which have been deployed on all SSRL macromolecular crystallography beamlines and several beamlines worldwide, are used by more than 80 research groups in remote locations, establishing a new paradigm for macromolecular crystallography experimentation.

• Research Organization: Stanford Linear Accelerator Center (SLAC)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC02-76SF00515
• OSTI ID: 953572
• Report Number(s): SLAC-REPRINT-2009-312
• Journal Information: Acta Crystallogr.D Biol.Crystallogr.D64:1210,2008, Journal Name: Acta Crystallogr.D Biol.Crystallogr.D64:1210,2008 Journal Issue: Pt12 Vol. 64; ISSN 0907-4449
• Country of Publication: United States
• Language: English

Related Subjects

43 PARTICLE ACCELERATORS
AUTOMATION
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CONTROL SYSTEMS
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CRYSTALLOGRAPHY
CRYSTALS
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DIFFRACTION
LIGHT SOURCES
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BIO
STANFORD LINEAR ACCELERATOR CENTER
SYNCHROTRON RADIATION