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The Stanford Automated Mounter: Pushing the limits of sample exchange at the SSRL macromolecular crystallography beamlines

Journal Article · · Journal of Applied Crystallography (Online)
 [1];  [1];  [1];  [1]
  1. SLAC National Accelerator Lab., Menlo Park, CA (United States)

The Stanford Automated Mounter System, a system for mounting and dismounting cryo-cooled crystals, has been upgraded to increase the throughput of samples on the macromolecular crystallography beamlines at the Stanford Synchrotron Radiation Lightsource. This upgrade speeds up robot maneuvers, reduces the heating/drying cycles, pre-fetches samples and adds an air-knife to remove frost from the gripper arms. As a result, sample pin exchange during automated crystal quality screening now takes about 25 s, five times faster than before this upgrade.

Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
USDOE Office of Science (SC)
Grant/Contract Number:
AC02-76SF00515
OSTI ID:
1253923
Report Number(s):
SLAC-PUB-16477; PII: S1600576716000649
Journal Information:
Journal of Applied Crystallography (Online), Journal Name: Journal of Applied Crystallography (Online) Journal Issue: 2 Vol. 49; ISSN 1600-5767; ISSN JACGAR
Publisher:
International Union of CrystallographyCopyright Statement
Country of Publication:
United States
Language:
English

References (13)

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Goniometer-based femtosecond crystallography with X-ray free electron lasers journal October 2014
An automated system to mount cryo-cooled protein crystals on a synchrotron beamline, using compact sample cassettes and a small-scale robot journal November 2002
Implementation and performance of SIBYLS: a dual endstation small-angle X-ray scattering and macromolecular crystallography beamline at the Advanced Light Source journal January 2013
Beamline automation and remote access at NSRRC BL-13 journal August 2008
New paradigm for macromolecular crystallography experiments at SSRL: automated crystal screening and remote data collection journal November 2008
Blu-Ice and the Distributed Control System : software for data acquisition and instrument control at macromolecular crystallography beamlines journal November 2002
High-throughput operation of sample-exchange robots with double tongs at the Photon Factory beamlines journal April 2008
Improvement of an automated protein crystal exchange system PAM for high-throughput data collection journal October 2013
Capture and X-ray diffraction studies of protein microcrystals in a microfluidic trap array journal March 2015
MX1: a bending-magnet crystallography beamline serving both chemical and macromolecular crystallography communities at the Australian Synchrotron journal January 2015
High-density grids for efficient data collection from multiple crystals journal January 2016

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