Direct electrochemistry and electrocatalysis of horseradish peroxidase immobilized in hybrid organic-inorganic film of chitosan/sol-gel/carbon nanotubes

Journal Article · Wed Apr 15 04:00:00 EDT 2009 · Talanta, 78(1):120-125

DOI:https://doi.org/10.1016/j.talanta.2008.10.063· OSTI ID:950727

Kang, Xinhuang; Wang, Jun; Tang, Zhiwen; Wu, Hong; Lin, Yuehe

A hybrid organic-inorganic nanocomposite film of chitosan/sol-gel/multi-walled carbon nanotubes was constructed for the immobilization of horseradish peroxidase (HRP). This film was characterized by scanning electron microscopy. Direct electron transfer (DET) and bioelectrocatalysis of HRP incorporated into the composite film were investigated. The results indicate that the film can provide a favorable microenvironment for HRP to perform DET on the surface of glassy carbon electrodes with a pair of quasi-reversible redox waves and to retain its bioelectrocatalytic activity toward hydrogen peroxide.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 950727
• Report Number(s): PNNL-SA-63346; 30493
• Journal Information: Talanta, 78(1):120-125, Journal Name: Talanta, 78(1):120-125 Journal Issue: 1 Vol. 78; ISSN 0039-9140; ISSN TLNTA2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CARBON
Direct electrochemistry
ELECTROCHEMISTRY
ELECTRODES
ELECTRON TRANSFER
Environmental Molecular Sciences Laboratory
HYDROGEN PEROXIDE
Horseradish peroxidase
NANOTUBES
PEROXIDASES
SCANNING ELECTRON MICROSCOPY