Automated Gain Control Ion Funnel Trap for Orthogonal Time-of-Flight Mass Spectrometry
Time-of-flight mass spectrometry (TOF MS) is increasingly used in proteomics research. Herein, we report on the development and characterization of an ultra-sensitive TOF MS instrument equipped with an electrodynamic ion funnel trap (IFT) that employs an automatic gain control (AGC) capability. The IFT-TOF MS was coupled to a reverse-phase capillary liquid chromatography (RPLC) separation and evaluated in experiments with complex proteolytic digests. When applied to a global tryptic digest of Shewanella oneidensis proteins, an order-of-magnitude increase in sensitivity compared to that of the conventional continuous mode of operation was achieved due to efficient ion accumulation prior to TOF MS analysis. As a result of this sensitivity improvement and related improvement in mass measurement accuracy, the number of unique peptides identified in the AGC-IFT mode was 5-fold greater than that obtained in the continuous mode.
- Research Organization:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 949105
- Report Number(s):
- PNNL-SA-59266; KP1501021; TRN: US200907%%397
- Journal Information:
- Analytical Chemistry, 80(14):5367-5376, Vol. 80, Issue 14
- Country of Publication:
- United States
- Language:
- English
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