Using Non-Invasive Multi-Spectral Imaging to Quantitatively Assess Tissue Vasculature

Journal Article · Thu Oct 04 04:00:00 EDT 2007 · Journal of Biomedical Optics, vol. 12, no. 5, November 1, 2007, pp. 051604

OSTI ID:943806

Vogel, A; Chernomordik, V; Riley, J; Hassan, M; Amyot, F; Dasgeb, B; Demos, S G; Pursley, R; Little, R; Yarchoan, R; Tao, Y; Gandjbakhche, A H

This research describes a non-invasive, non-contact method used to quantitatively analyze the functional characteristics of tissue. Multi-spectral images collected at several near-infrared wavelengths are input into a mathematical optical skin model that considers the contributions from different analytes in the epidermis and dermis skin layers. Through a reconstruction algorithm, we can quantify the percent of blood in a given area of tissue and the fraction of that blood that is oxygenated. Imaging normal tissue confirms previously reported values for the percent of blood in tissue and the percent of blood that is oxygenated in tissue and surrounding vasculature, for the normal state and when ischemia is induced. This methodology has been applied to assess vascular Kaposi's sarcoma lesions and the surrounding tissue before and during experimental therapies. The multi-spectral imaging technique has been combined with laser Doppler imaging to gain additional information. Results indicate that these techniques are able to provide quantitative and functional information about tissue changes during experimental drug therapy and investigate progression of disease before changes are visibly apparent, suggesting a potential for them to be used as complementary imaging techniques to clinical assessment.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 943806
• Report Number(s): UCRL-JRNL-235502
• Journal Information: Journal of Biomedical Optics, vol. 12, no. 5, November 1, 2007, pp. 051604, Journal Name: Journal of Biomedical Optics, vol. 12, no. 5, November 1, 2007, pp. 051604 Journal Issue: 5 Vol. 12; ISSN JBOPFO
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD
DISEASES
EPIDERMIS
FUNCTIONALS
ISCHEMIA
LASERS
SARCOMAS
THERAPY
WAVELENGTHS