Studies in Solid Phase Peptide Synthesis: A Personal Perspective

Journal Article · Fri Jun 01 04:00:00 EDT 2007 · Biopolymers:Peptide Science, vol. 90, no. 3, July 23, 2007, pp. 215 - 233

OSTI ID:942028

Mitchell, A R

By the early 1970s it had became apparent that the solid phase synthesis of ribonuclease A could not be generalized. Consequently, virtually every aspect of solid phase peptide synthesis (SPPS) was reexamined and improved during the decade of the 1970s. The sensitive detection and elimination of possible side reactions (amino acid insertion, N{sup {alpha}}-trifluoroacetylation, N{sup {alpha}{var_epsilon}}-alkylation) was examined. The quantitation of coupling efficiency in SPPS as a function of chain length was studied. A new and improved support for SPPS, the 'PAM-resin', was prepared and evaluated. These and many other studies from the Merrifield laboratory and elsewhere increased the general acceptance of SPPS leading to the 1984 Nobel Prize in Chemistry for Bruce Merrifield.

• Research Organization: Lawrence Livermore National Laboratory (LLNL), Livermore, CA
• Sponsoring Organization: USDOE
• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 942028
• Report Number(s): UCRL-JRNL-231463
• Journal Information: Biopolymers:Peptide Science, vol. 90, no. 3, July 23, 2007, pp. 215 - 233, Journal Name: Biopolymers:Peptide Science, vol. 90, no. 3, July 23, 2007, pp. 215 - 233 Journal Issue: 3 Vol. 90
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
CHAINS
CHEMISTRY
DETECTION
EFFICIENCY
PEPTIDES
RNA-ASE
SYNTHESIS