A novel fermentation pathway in an Escherichia coli mutant producing succinic acid, acetic acid, and ethanol.
Escherichia coli strain NZN111, which is unable to grow fermentatively because of insertional inactivation of the genes encoding pyruvate: formate lyase and the fermentative lactate dehydrogenase, gave rise spontaneously to a chromosomal mutation that restored its ability to ferment glucose. The mutant strain, named AFP111, fermented glucose more slowly than did its wild-type ancestor, strain W1485, and generated a very different spectrum of products. AFP111 produced succinic acid, acetic acid, and ethanol in proportions of approx 2:1:1. Calculations of carbon and electron balances accounted fully for the observed products; 1 mol of glucose was converted to 1 mol of succinic acid and 0.5 mol each of acetic acid and ethanol. The data support the emergence in E.coli of a novel succinic acid:acetic acid:ethanol fermentation pathway.
- Research Organization:
- Argonne National Laboratory (ANL)
- Sponsoring Organization:
- ER
- DOE Contract Number:
- AC02-06CH11357
- OSTI ID:
- 938297
- Report Number(s):
- ANL/ER/JA-29109
- Journal Information:
- Appl. Biochem. Biotechnol., Journal Name: Appl. Biochem. Biotechnol. Journal Issue: Spring 1998 Vol. 70-72; ISSN ABIBDL; ISSN 0273-2289
- Country of Publication:
- United States
- Language:
- ENGLISH
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Mutant E. coli strain with increased succinic acid production
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