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Title: A novel fermentation pathway in an Escherichia coli mutant producing succinic acid, acetic acid, and ethanol.

Abstract

Escherichia coli strain NZN111, which is unable to grow fermentatively because of insertional inactivation of the genes encoding pyruvate: formate lyase and the fermentative lactate dehydrogenase, gave rise spontaneously to a chromosomal mutation that restored its ability to ferment glucose. The mutant strain, named AFP111, fermented glucose more slowly than did its wild-type ancestor, strain W1485, and generated a very different spectrum of products. AFP111 produced succinic acid, acetic acid, and ethanol in proportions of approx 2:1:1. Calculations of carbon and electron balances accounted fully for the observed products; 1 mol of glucose was converted to 1 mol of succinic acid and 0.5 mol each of acetic acid and ethanol. The data support the emergence in E.coli of a novel succinic acid:acetic acid:ethanol fermentation pathway.

Authors:
; ; ; ;
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
ER
OSTI Identifier:
938297
Report Number(s):
ANL/ER/JA-29109
Journal ID: ISSN 0273-2289; ABIBDL; TRN: US200908%%274
DOE Contract Number:  
DE-AC02-06CH11357
Resource Type:
Journal Article
Journal Name:
Appl. Biochem. Biotechnol.
Additional Journal Information:
Journal Volume: 70-72; Journal Issue: Spring 1998; Journal ID: ISSN 0273-2289
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; ACETIC ACID; ESCHERICHIA COLI; ETHANOL; FERMENTATION; GLUCOSE; LACTATE DEHYDROGENASE; MUTANTS; SUCCINIC ACID

Citation Formats

Donnelly, M I, Millard, C S, Clark, D P, Chen, M J, Rathke, J W, and Southern Illinois Univ. A novel fermentation pathway in an Escherichia coli mutant producing succinic acid, acetic acid, and ethanol.. United States: N. p., 1998. Web. doi:10.1007/BF02920135.
Donnelly, M I, Millard, C S, Clark, D P, Chen, M J, Rathke, J W, & Southern Illinois Univ. A novel fermentation pathway in an Escherichia coli mutant producing succinic acid, acetic acid, and ethanol.. United States. https://doi.org/10.1007/BF02920135
Donnelly, M I, Millard, C S, Clark, D P, Chen, M J, Rathke, J W, and Southern Illinois Univ. 1998. "A novel fermentation pathway in an Escherichia coli mutant producing succinic acid, acetic acid, and ethanol.". United States. https://doi.org/10.1007/BF02920135.
@article{osti_938297,
title = {A novel fermentation pathway in an Escherichia coli mutant producing succinic acid, acetic acid, and ethanol.},
author = {Donnelly, M I and Millard, C S and Clark, D P and Chen, M J and Rathke, J W and Southern Illinois Univ.},
abstractNote = {Escherichia coli strain NZN111, which is unable to grow fermentatively because of insertional inactivation of the genes encoding pyruvate: formate lyase and the fermentative lactate dehydrogenase, gave rise spontaneously to a chromosomal mutation that restored its ability to ferment glucose. The mutant strain, named AFP111, fermented glucose more slowly than did its wild-type ancestor, strain W1485, and generated a very different spectrum of products. AFP111 produced succinic acid, acetic acid, and ethanol in proportions of approx 2:1:1. Calculations of carbon and electron balances accounted fully for the observed products; 1 mol of glucose was converted to 1 mol of succinic acid and 0.5 mol each of acetic acid and ethanol. The data support the emergence in E.coli of a novel succinic acid:acetic acid:ethanol fermentation pathway.},
doi = {10.1007/BF02920135},
url = {https://www.osti.gov/biblio/938297}, journal = {Appl. Biochem. Biotechnol.},
issn = {0273-2289},
number = Spring 1998,
volume = 70-72,
place = {United States},
year = {1998},
month = {4}
}