The relative radiosensitivity of TK6 and W1-L2-NS lymphoblastoid cells derived from a common source is primarily determined by their p53 mutational status.
The lymphoblastoid cell lines WI-L2-NS and TK6 were derived from a non-clonal pool of cells taken from a human spleen. Despite their common background they exhibit marked differences in radiosensitivities; D{sub 0} values of 93 and 67 cGy have been reported for WI-L2-NS and TK6 cells respectively. We show here that this differential radiosensitivity is due to a decreased ability of the WI-L2-NS cell line to undergo radiation-induced apoptosis. Further, the WI-L2-NS cell line overexpresses the p53 gene product as a result of a mutation in codon 237 of the p53 gene. These data indicate that WI-L2-NS cells through disruption of normal p53 function are unable to engage the radiation-induced apoptosis program and so are relatively radioresistant.
- Research Organization:
- Argonne National Laboratory (ANL)
- Sponsoring Organization:
- ER
- DOE Contract Number:
- AC02-06CH11357
- OSTI ID:
- 937741
- Report Number(s):
- ANL/CMB/JA-16802
- Journal Information:
- Mutat. Res. Lett., Journal Name: Mutat. Res. Lett. Journal Issue: 2 ; Feb. 1995 Vol. 346; ISSN 0165-7992; ISSN MRLEDH
- Country of Publication:
- United States
- Language:
- ENGLISH
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