Involvement of recombination in x-ray mutagenesis of human cells
- Los Alamos National Lab., NM (United States)
- Harvard School of Public Health, Boston, MA (United States)
Closely related human lymphoblastoid cell lines derived from WI-L2 differ greatly in their responses to X-irradiation. Compared with TK6 (ATCC CRL 8015), WI-L2-NS (ATCC CRL 8155) has an enhanced X-ray survival. The induction of mutation by X-rays is also markedly different. The hemizygous hprt locus is slightly more mutable in WI-L2-NS than in TK6, and the dose response fits best to a linear-quadratic curve rather than the linear fit of TK6X-ray induced mutation at the autosomal tk locus in heterozygotes derived from WI-L2-NS is 20-50 fold higher than in heterozygotes derived from TK6. A larger proportion of WI-L2-NS mutants had lost heterozygosity compared with mutants of TK6. , Fluorescence in situ hybridization indicated that loss of heterozygosity was due almost uniformly to deletion of an allele in mutants of TK6, and to recombination or gene conversion in mutants of WI-L2-NS. These results indicate that recombinational repair contributes to both cell survival and mutation following exposure to ionizing radiation.
- Research Organization:
- Los Alamos National Lab., NM (United States)
- Sponsoring Organization:
- DOE; USDOE, Washington, DC (United States)
- DOE Contract Number:
- W-7405-ENG-36
- OSTI ID:
- 7368604
- Report Number(s):
- LA-UR-93-1696; CONF-930454--2; ON: DE93014448
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
ELECTROMAGNETIC RADIATION
ENZYMES
GENE MUTATIONS
GENE RECOMBINATION
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
GLYCOSYL TRANSFERASES
HYPOXANTHINE PHOSPHORIBOSYLTRANSFERASE
IONIZING RADIATIONS
LEUKOCYTES
LYMPHOCYTES
MATERIALS
MUTAGENESIS
MUTATIONS
ORGANIC COMPOUNDS
PENTOSYL TRANSFERASES
PROTEINS
RADIATION EFFECTS
RADIATIONS
SOMATIC CELLS
SPLEEN CELLS
TRANSFERASES
X RADIATION