Elastomeric Microchip Electrospray Emitter for Stable Cone-Jet Mode Operation in the Nanoflow Regime.

Journal Article · Thu May 15 04:00:00 EDT 2008 · Analytical Chemistry, 80(10):3824-3831

DOI:https://doi.org/10.1021/ac8000786· OSTI ID:937029

Kelly, Ryan T.; Tang, Keqi; Irimia, Daniel; Toner, Mehmet; Smith, Richard D.

Despite widespread interest in applying lab-on-a-chip technologies to mass spectrometry (MS)-based analyses, the coupling of microfluidics to electrospray ionization (ESI)-MS remains challenging. We report a robust, integrated poly(dimethylsiloxane) microchip interface for ESI-MS using simple and widely accessible microfabrication procedures. The interface uses an auxiliary channel to provide electrical contact in the Taylor cone of the electrospray without sample loss or dilution. The electric field at the channel terminus is enhanced by two vertical cuts that cause the interface to taper to a line rather than to a point, and the formation of small Taylor cones at the channel exit ensures sub-nL post-column dead volumes. While comparable ESI-MS sensitivities were achieved using both microchip and conventional fused silica capillary emitters, stable cone-jet mode electrospray could be established over a far broader range of flow rates (from 50–1000 nL/min) and applied potentials using the microchip emitters. This special feature of the microchip emitter should minimize the fine tuning required for electrospray optimization and make the stable electrospray more resistant to external perturbations.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 937029
• Report Number(s): PNNL-SA-58551; KP1601010
• Journal Information: Analytical Chemistry, 80(10):3824-3831, Journal Name: Analytical Chemistry, 80(10):3824-3831 Journal Issue: 10 Vol. 80
• Country of Publication: United States
• Language: English