High-throughput proteomics of breast carcinoma cells: a focus on FTICR-MS
Journal Article
·
· Expert Review of Proteomics, 2008 Jun;5(3):445-55
Discovery of better biomarkers for diagnosis, prognosis, and therapy-response prediction is the most critical task of a scientific quest aimed at developing newly designed, tailor-made therapies for patients with cancer. Consequently, a proteome wide analysis, in addition to genomic studies, is an absolute requirement for a complete functional understanding of tumor biology. Ultra-sensitive, high-performance Fourier transform ion cyclotron resonance (FTICR) mass spectrometry (MS) currently holds an important role in fulfilling the demands of biomarker discovery. In this review, we describe the applicability of FTICR MS for breast cancer proteomics, particularly for the analysis of complex protein mixtures obtained from a limited number of cells typically available from clinical specimens.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 936208
- Report Number(s):
- PNNL-SA-59969; 400412000
- Journal Information:
- Expert Review of Proteomics, 2008 Jun;5(3):445-55, Journal Name: Expert Review of Proteomics, 2008 Jun;5(3):445-55 Journal Issue: 3 Vol. 5
- Country of Publication:
- United States
- Language:
- English
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