Targeted Tandem Mass Spectrometry for High-Throughput Comparative Proteomics Employing NanoLC-FTICR MS with External Ion Dissociation

Journal Article · Thu May 03 04:00:00 EDT 2007 · Journal of the American Society for Mass Spectrometry, 18(7):1332-1343

DOI:https://doi.org/10.1016/j.jasms.2007.04.011· OSTI ID:917941

Kang, Hyuk; Pasa-Tolic, Liljiana; Smith, Richard D

ABSTRACT-Targeted tandem mass spectrometry (MS/MS) is an attractive proteomic approach that allows selective identification of peptides exhibiting abundance differences between culture conditions and/or diseased states. Herein, we report on a targeted LC-MS/MS capability realized with a 7 Tesla Fourier transform ion cyclotron resonance (FTICR) mass spectrometer equipped with a quadrupole interface that provides data-dependent ion selection, accumulation, and dissociation externally to the ICR trap. Identification of a subset of differentially abundant proteins from Shewanella oneidensis grown under suboxic vs. aerobic conditions demonstrates the feasibility of such approach. High mass resolution offered by FTICR and effective on-the-fly elution time correction facilitated accurate selection of targets, while high mass measurement accuracy MS/MS data resulted in unambiguous peptide identifications.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 917941
• Report Number(s): PNNL-SA-54095; 17797; KP1501021
• Journal Information: Journal of the American Society for Mass Spectrometry, 18(7):1332-1343, Journal Name: Journal of the American Society for Mass Spectrometry, 18(7):1332-1343 Journal Issue: 7 Vol. 18; ISSN JAMSEF; ISSN 1044-0305
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
ABUNDANCE
ACCURACY
AEROBIC CONDITIONS
DISSOCIATION
Environmental Molecular Sciences Laboratory
FTICR
ION CYCLOTRON-RESONANCE
MASS RESOLUTION
MASS SPECTROMETERS
MASS SPECTROSCOPY
PEPTIDES
PROTEINS
QUADRUPOLES
TARGETS
comparative proteomics
data-dependent MS/MS
targeted MS/MS