Response of Desulfovibrio vulgaris to Alkaline Stress
The response of exponentially growing Desulfovibrio vulgarisHildenborough to pH 10 stress was studied using oligonucleotidemicroarrays and a study set of mutants with genes suggested by microarraydata to be involved in the alkaline stress response deleted. The datashowed that the response of D. vulgaris to increased pH is generallysimilar to that of Escherichia coli but is apparently controlled byunique regulatory circuits since the alternative sigma factors (sigma Sand sigma E) contributing to this stress response in E. coli appear to beabsent in D. vulgaris. Genes previously reported to be up-regulated in E.coli were up-regulated in D. vulgaris; these genes included three ATPasegenes and a tryptophan synthase gene. Transcription of chaperone andprotease genes (encoding ATP-dependent Clp and La proteases and DnaK) wasalso elevated in D. vulgaris. As in E. coli, genes involved in flagellumsynthesis were down-regulated. The transcriptional data also identifiedregulators, distinct from sigma S and sigma E, that are likely part of aD. vulgaris Hildenborough-specific stress response system.Characterization of a study set of mutants with genes implicated inalkaline stress response deleted confirmed that there was protectiveinvolvement of the sodium/proton antiporter NhaC-2, tryptophanase A, andtwo putative regulators/histidine kinases (DVU0331 andDVU2580).
- Research Organization:
- COLLABORATION - UWA
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC02-05CH11231
- OSTI ID:
- 928727
- Report Number(s):
- LBNL--63622; BnR: KP1102010
- Journal Information:
- Journal of Bacteriology, Journal Name: Journal of Bacteriology Journal Issue: 24 Vol. 189; ISSN JOBAAY; ISSN 0021-9193
- Country of Publication:
- United States
- Language:
- English
Similar Records
Global Analysis of Heat Shock Response in Desulfovibrio vulgaris Hildenborough.
Complementation of an Escherichia coli pyrF mutant with DNA from Desulfovibrio vulgaris