Complementation of an Escherichia coli pyrF mutant with DNA from Desulfovibrio vulgaris
Journal Article
·
· J. Bacteriol.; (United States)
OSTI ID:5896130
A PyrF/sup -/ mutant of Escherichia coli (SK1108, pyrF::Tn5 Kan/sup r/) was complemented with the Desulfovibrio vulgaris (Hildenborough) structural gene for orotidine-5'-phosphate decarboxylase. Either orientation of a 1.6-kilobase-pair D. vulgaris DNA fragment (pLP3B or pLP3A) complemented the PyrF/sup -/ strain suggesting that the D. vulgaris pyrF promoter was functional. The apparent product of the D. vulgaris pyrF gene was a single 26-kilodalton polypeptide. These results demonstrate the utility of E. coli cloning systems in studying metabolic and energetic pathways in sulfate-reducing bacteria.
- Research Organization:
- Univ. of Georgia, Athens
- OSTI ID:
- 5896130
- Journal Information:
- J. Bacteriol.; (United States), Journal Name: J. Bacteriol.; (United States) Vol. 165:2; ISSN JOBAA
- Country of Publication:
- United States
- Language:
- English
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