Capillary-Based Multi Nanoelectrospray Emitters: Improvements in Ion Transmission Efficiency and Implementation with Capillary Reversed-Phase LC-ESI-MS
Journal Article
·
· Analytical Chemistry, 80(1):143-149
We describe the coupling of liquid chromatography (LC) separations with mass spectrometry (MS) using nanoelectrospray ionization (nanoESI) multi-emitters. The array of 19 emitters reduced the flow rate delivered to each emitter, allowing the enhanced sensitivity that is characteristic of nanoESI to be extended to higher flow rate separations. The signal for peptides from spiked proteins in a human plasma tryptic digest increased 11-fold on average when the multi-emitters were employed, due to increased ionization efficiency and improved ion transfer efficiency through a newly designed heated multi-capillary MS inlet. Additionally, the LC peak signal-to-noise ratio increased ~7-fold when the multi-emitter configuration was used. The low dead volume of the emitter arrays preserved peak shape and resolution for robust capillary LC separations using total flow rates of 2-µL/min.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 923231
- Report Number(s):
- PNNL-SA-56420; 20492a; 400412000
- Journal Information:
- Analytical Chemistry, 80(1):143-149, Journal Name: Analytical Chemistry, 80(1):143-149 Journal Issue: 1 Vol. 80
- Country of Publication:
- United States
- Language:
- English
Similar Records
Array of Chemically Etched Fused Silica Emitters for Improving the Sensitivity and Quantitation of Electrospray Ionization Mass Spectrometry
MicroSPE-nanoLC-ESI-MS/MS Using 10-μm-i.d. Silica-Based Monolithic Columns for Proteomics
Capillary Isotachophoresis-Nanoelectrospray Ionization-Selected Reaction Monitoring MS via a Novel Sheathless Interface for High Sensitivity Sample Quantification
Journal Article
·
Fri Jun 01 00:00:00 EDT 2007
· Analytical Chemistry, 79(11):4192-4198
·
OSTI ID:910258
MicroSPE-nanoLC-ESI-MS/MS Using 10-μm-i.d. Silica-Based Monolithic Columns for Proteomics
Journal Article
·
Sun Dec 31 23:00:00 EST 2006
· Analytical Chemistry
·
OSTI ID:898092
Capillary Isotachophoresis-Nanoelectrospray Ionization-Selected Reaction Monitoring MS via a Novel Sheathless Interface for High Sensitivity Sample Quantification
Journal Article
·
Fri Jun 21 00:00:00 EDT 2013
· Analytical Chemistry, 85(15):7308-7315
·
OSTI ID:1091482