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Method for using a yeast alpha-amylase promoter

Patent ·
OSTI ID:921317
 [1];  [2];  [3];  [4]
  1. Richland, WA
  2. Pendleton, OR
  3. Kennewick, WA
  4. Pasco, WA
+ Show Author Affiliations
The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA
Sponsoring Organization:
United States Department of Energy
DOE Contract Number:
AC06-76RL01830
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Number(s):
6,551,798
Application Number:
09/921,942
OSTI ID:
921317
Country of Publication:
United States
Language:
English

References (20)

Kinetics of Growth and Amylase Production of Saccharomycopsis fibuligera on Potato Processing Wastewater † journal January 1980
New Amylolytic Yeast Strains for Starch and Dextrin Fermentation journal January 1988
Direct alcohol fermentation of starch by a derepressed mutant ofSchwanniomyces castellii journal January 1994
ISOLATION AND CHARACTERISATION OF THE AMYLOTIC SYSTEM OF SCHWANNIOMYCES CASTELLII journal September 1984
Production of Amylolytic Enzymes by Several Yeast Species journal September 1982
Molecular structure of the SWA2 gene encoding an AMY1-related ?-amylase from Schwanniomyces occidentalis journal July 1993
The Molecular Biology of Schwanniomyces occidentalis Klocker journal January 1999
Optimization of single-cell-protein production from cassava starch using Schwanniomyces castellii journal November 1995
Cloning of α-amylase gene fromSchwanniomyces occidentalis and expression inSaccharomyces cerevisiae journal December 1998
High-efficiency, one-step starch utilization by transformed Saccharomyces cells which secrete both yeast glucoamylase and mouse alpha-amylase. journal January 1988
High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris journal May 1989
Isolation of Yeast DNA book February 1996
Fermentation of starch to ethanol by a co-culture of Saccharomycopsis fibuligera and Saccharomyces cerevisiae journal May 1993
Recent Advances in the Expression of Foreign Genes in Pichia pastoris journal August 1993
Replacement of a Metabolic Pathway for Large-Scale Production of Lactic Acid from Engineered Yeasts journal September 1999
A DNA fragment containing the ADE2 gene from Schwanniomyces occidentalis can be maintained as an extrachromosomal element journal January 1991
Analysis of the alpha-amylase gene of Schwanniomyces occidentalis and the secretion of its gene product in transformants of different yeast genera journal October 1989
Cloning of the Schwanniomyces occidentalis glucoamylase gene (GAM1) and its expression in Saccharomyces cerevisiae journal October 1990
GUS fusions: beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants. journal December 1987
Expression and regulation of glucoamylase from the yeast Schwanniomyces castellii. journal January 1990

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