Production of alpha-amylase by yeast

Journal Article · Thu Jan 01 04:00:00 EST 1987 · CRC Crit. Rev. Biotechnol.; (United States)

DOI:https://doi.org/10.3109/07388558709086979· OSTI ID:5564623

Thomse, K K

The enzyme alpha-amylase confers to an organism the enzymatic activity for the degradation of polyglucosides with alpha-1,4 glycosidic bonds such as starch and glycogen which are among the major storage compounds in plants and animals. Most alpha-amylases are single polypeptides of molecular weights around 50,000 dalton. They are generally found in the digestive tract of animals and in germinating seeds. Among the products released upon enzymatic degradation of polyglucosides maltose, a sugar that can be utilized as carbon source by yeast, is a major constituent. A cDNA segment complementary to mouse salivary amylase messenger RNA has been inserted into the yeast expression vector pMA56 behind the promoter of the gene encoding alcohol dehydrogenase I of yeast. Yeast transformants harboring plasmids with the normal orientation of the promoter and the mouse amylase cDNA gene produce amylase and release the enzyme in free form into the culture medium. Approximately 90% of the amylase activity is found in the medium. Yeast strains carrying MAL allele and transformed with a plasmid which directed the synthesis of mouse alpha-amylase were tested on plates containing starch and in batch fermentations using different high molecular weight sugars and oligosaccharides as carbon source. The results of these experiments will be discussed. (Refs. 21).

• Research Organization: Univ. Aarhus, Aarhus, Denmark. Dept. Physiology, Carlsberg Lab., Valby, Denmark
• OSTI ID: 5564623
• Journal Information: CRC Crit. Rev. Biotechnol.; (United States), Journal Name: CRC Crit. Rev. Biotechnol.; (United States) Vol. 5:3; ISSN CRBTE
• Country of Publication: United States
• Language: English

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59 BASIC BIOLOGICAL SCIENCES
AMYLASE
ANIMALS
BIOLOGY
BODY
CELL CONSTITUENTS
CLONING
DNA
DNA-CLONING
ENZYMES
FUNGI
GENETIC ENGINEERING
GENETICS
GLANDS
GLYCOSYL HYDROLASES
HYDROLASES
MAMMALS
MICE
MICROORGANISMS
NUCLEIC ACIDS
O-GLYCOSYL HYDROLASES
ORGANIC COMPOUNDS
ORGANS
PLANTS
PLASMIDS
PRODUCTION
RODENTS
SALIVARY GLANDS
VERTEBRATES
YEASTS