Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

Bavykin, Sergei G

Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

Patent · Mon Dec 03 23:00:00 EST 2007

OSTI ID:920673

Bavykin, Sergei G ^[1]

Darien, IL

The present invention relates to methods and compositions for using nucleotide sequence variations of 16S and 23S rRNA within the B. cereus group to discriminate a highly infectious bacterium B. anthracis from closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations and discriminate B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed samples, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL

Sponsoring Organization:: United States Department of Energy

DOE Contract Number:: W-31109-ENG-38

Assignee:: The University of Chicago (Chicago, IL)

Patent Number(s):: 7,303,874

Application Number:: 10/647,423

OSTI ID:: 920673

Country of Publication:: United States

Language:: English

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