Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

Bavykin, Sergei G; Mirzabekov, Andrei D

Discrimination of Bacillus anthracis from closely related microorganisms by analysis of 16S and 23S rRNA with oligonucleotide microchips

Patent · Tue Oct 30 04:00:00 EDT 2007

OSTI ID:919056

Bavykin, Sergei G ^[1]; Mirzabekov, Andrei D ^[2]

Darien, IL
Moscow, RU

The present invention is directed to a novel method of discriminating a highly infectious bacterium Bacillus anthracis from a group of closely related microorganisms. Sequence variations in the 16S and 23S rRNA of the B. cereus subgroup including B. anthracis are utilized to construct an array that can detect these sequence variations through selective hybridizations. The identification and analysis of these sequence variations enables positive discrimination of isolates of the B. cereus group that includes B. anthracis. Discrimination of single base differences in rRNA was achieved with a microchip during analysis of B. cereus group isolates from both single and in mixed probes, as well as identification of polymorphic sites. Successful use of a microchip to determine the appropriate subgroup classification using eight reference microorganisms from the B. cereus group as a study set, was demonstrated.

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Research Organization:: Argonne National Laboratory (ANL), Argonne, IL

Sponsoring Organization:: United States Department of Energy

DOE Contract Number:: W-31109-ENG-38

Assignee:: The University of Chicago (Chicago, IL)

Patent Number(s):: 7,288,371

Application Number:: 10/287,455

OSTI ID:: 919056

Country of Publication:: United States

Language:: English

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