Fluorescense Anisotropy Studies of Molecularly Imprinted Polymer Sensors

Journal Article · Wed Aug 03 04:00:00 EDT 2005 · Luminescence, 21(1):7-14

DOI:https://doi.org/10.1002/bio.874· OSTI ID:920209

Chen, Yin-Chu; Wang, Zheming; Yan, Mingdi; Prahl, Scott A

Molecularly imprinted polymers (MIPs) are used as recognition elements in biochemical sensors. In a fluorescence-based MIP sensor system, it is difficult to distinguish the analyte fluorescence from the background fluorescence of the polymer itself. We studied steady-state fluorescence anisotropy of anthracene imprinted in a polymer (polyurethane) matrix. Vertically polarized excitation light was incident on MIP films coated on silicon wafers; vertically and horizontally polarized emission was measured. We compared the fluorescence anisotropy of MIPs with imprinted molecules, MIPs with the imprinted molecules extracted, MIPs with rebound molecules, and nonimprinted control polymers (without binding cavities). It is shown that differences in fluorescence anisotropy between the polymers and imprinted fluorescent molecules may provide a means to discriminate the fluorescence of analyte from that of the background polymer.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 920209
• Report Number(s): PNNL-SA-40194; 3532; KP1704020
• Journal Information: Luminescence, 21(1):7-14, Journal Name: Luminescence, 21(1):7-14 Journal Issue: 1 Vol. 21
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
59 BASIC BIOLOGICAL SCIENCES
ANISOTROPY
ANTHRACENE
BIOCHEMISTRY
CAVITIES
EXCITATION
Environmental Molecular Sciences Laboratory
FLUORESCENCE
POLYURETHANES
SENSORS
polarization
ployurethane
anthracene
polystyrene
dansyl-adenine