Fluorescence Anisotropy Studies of Molecularly Imprinted Polymers

Journal Article · Sun Jan 01 04:00:00 EST 2006 · Luminescence, 21(1):7-14

DOI:https://doi.org/10.1002/bio.874· OSTI ID:878268

Chen, Yin-Chu; Wang, Zheming; Yan, Mingdi; Prahl, Scott A

A molecularly imprinted polymer (MIP) is a biomimetic material that can be used as a biochemical sensing element. We studied the steady-state and time-resolved fluorescence and fluorescence anisotropy of anthracene imprinted polyurethane. We compared MIPs with imprinted analytes present, MIPs with the imprinted analytes extracted, MIPs with rebound analytes, non-imprinted control polymers (non-MIPs), and non-MIPs bound with analytes to understand MIP’s binding behavior. MIPs and non-MIPs had similar steady-state fluorescence anisotropy in the range of 0.11–0.24. Anthracene rebound in MIPs and non-MIPs had a fluorescence lifetime _=0.64 ns and a rotational correlation time _F =1.2–1.5 ns, both of which were shorter than that of MIPs with imprinted analytes present (_=2.03 ns and _F =2.7 ns). The steady-state anisotropy of polymer solutions increased exponentially with polymerization time and might be used to characterize the polymerization extent in-situ.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 878268
• Report Number(s): PNNL-SA-48527; 3532; KP1704020
• Journal Information: Luminescence, 21(1):7-14, Journal Name: Luminescence, 21(1):7-14 Journal Issue: 1 Vol. 21
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
ANISOTROPY
ANTHRACENE
Environmental Molecular Sciences Laboratory
FLUORESCENCE
POLYMERIZATION
POLYURETHANES