Surface chemistries for antibody microarrays
Journal Article
·
· Frontiers in Bioscience, 12:3956-3964
Enzyme-linked immunosorbent assay (ELISA) microarrays promise to be a powerful tool for the detection of disease biomarkers. The original technology for printing ELISA microarray chips and capturing antibodies on slides was derived from the DNA microarray field. However, due to the need to maintain antibody structure and function when immobilized, surface chemistries used for DNA microarrays are not always appropriate for ELISA microarrays. In order to identify better surface chemistries for antibody capture, a number of commercial companies and academic research groups have developed new slide types that could improve antibody function in microarray applications. In this review we compare and contrast the commercially available slide chemistries, as well as highlight some promising recent advances in the field.
- Research Organization:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (US)
- Sponsoring Organization:
- USDOE
- DOE Contract Number:
- AC05-76RL01830
- OSTI ID:
- 914677
- Report Number(s):
- PNNL-SA-54458; 400412000
- Journal Information:
- Frontiers in Bioscience, 12:3956-3964, Journal Name: Frontiers in Bioscience, 12:3956-3964 Vol. 12
- Country of Publication:
- United States
- Language:
- English
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