Genomic sequence of the xylose fermenting, insect-inhabitingyeast, Pichia stipitis

Journal Article · Mon Jun 25 04:00:00 EDT 2007 · Nature Biotech

DOI:https://doi.org/10.1038/nbt1290· OSTI ID:914506

Jeffries, Thomas W; Grigoriev, Igor; Grimwood, Jane; Laplaza, Jose M; Aerts, Andrea; Salamov, Asaf; Schmutz, Jeremy; Lindquist, Erika; Dehal, Paramvir; Shapiro, Harris; Jin, Yong-Su; Passoth, Volkmar; Richardson, Paul M

Xylose is a major constituent of angiosperm lignocellulose,so its fermentation is important for bioconversion to fuels andchemicals. Pichia stipitis is the best-studied native xylose fermentingyeast. Genes from P. stipitis have been used to engineer xylosemetabolism in Saccharomycescerevisiae, and the regulation of the P.stipitis genome offers insights into the mechanisms of xylose metabolismin yeasts. We have sequenced, assembled and finished the genome ofP.stipitis. As such, it is one of only a handful of completely finishedeukaryotic organisms undergoing analysis and manual curation. Thesequence has revealed aspects of genome organization, numerous genes forbiocoversion, preliminary insights into regulation of central metabolicpathways, numerous examples of co-localized genes with related functions,and evidence of how P. stipitis manages to achieve redox balance whilegrowing on xylose under microaerobic conditions.

• Research Organization: Ernest Orlando Lawrence Berkeley NationalLaboratory, Berkeley, CA (US)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 914506
• Report Number(s): LBNL--62457; BnR: KP1103010
• Journal Information: Nature Biotech, Journal Name: Nature Biotech Vol. 25
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOCONVERSION
BIOLOGICAL PATHWAYS
ENGINEERS
FERMENTATION
GENES
MAGNOLIOPHYTA
METABOLISM
REGULATIONS
XYLOSE
YEASTS
yeast genome xylose EST expression glycoside hydrolasetranscription factories