Hydrazine Determination in Sludge Samples by High Performance Liquid Chromatography
A high-performance liquid chromatographic method using ultraviolet (UV) detection was developed to detect and quantify hydrazine in a variety of environmental matrices. The method was developed primarily for sludge samples, but it is also applicable to soil and water samples. The hydrazine in the matrices was derivatized to their hydrazones with benzaldehyde. The derivatized hydrazones were separated using high performance liquid chromatography (HPLC) with a reversed-phase C-18 column in an isocratic mode with methanol-water (95:5, v/v), and detected with UV detection at 313 nm. The detection limit (25 ml) for the new analytical method is 0.0067 mg ml-1of hydrazine. Hydrazine showed low recovery in soil samples because components in soil oxidized hydrazine. Sludge samples that contained relatively high soil content also showed lower recovery. The technique is relatively simple and cost-effective, and is applicable for hydrazine analysis in different environmental matrices.
- Research Organization:
- Idaho National Lab. (INL), Idaho Falls, ID (United States)
- Sponsoring Organization:
- INEEL
- DOE Contract Number:
- DE-AC07-99ID-13727
- OSTI ID:
- 912298
- Report Number(s):
- INEEL/JOU-04-02155; TRN: US200801%%736
- Journal Information:
- Journal of Separation Science, Vol. 29, Issue 3
- Country of Publication:
- United States
- Language:
- English
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