Design of Instrumentation for Probing Changes in Electrospray Droplets Via the Stern-Volmer Relationship

Journal Article · Tue Feb 01 04:00:00 EST 2005 · Review of Scientific Instrumentation

DOI:https://doi.org/10.1063/1.1823191· OSTI ID:912272

Ham, Jason E; Durham, Bill; Scott, Jill R

Electrospray ionization is a proven method for introducing large intact molecules into the gas phase. However, the processes that occur within this method are poorly understood. We have developed instrumentation and methodology to probe the evolution of droplets within the electrospray plume. Using laser-induced fluorescence, emission lifetimes of [Ru(bpy)3]2+ with a known quencher of the ruthenium excited state, 2,3,5,6-tetramethyl-p-phenlyenediamine, were observed. Lifetimes were shown to decrease as quencher concentration increased, as expected. Rate constants of the ruthenium complex with and without quencher were also determined and correlated with the Stern-Volmer relationship. Stern-Volmer plots reveal the linearity of the quenching reaction and can be used to determine the relative concentrations of species within the electrosprayed droplets.

• Research Organization: Idaho National Laboratory (INL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC07-99ID13727
• OSTI ID: 912272
• Report Number(s): INEEL/JOU-04-01855
• Journal Information: Review of Scientific Instrumentation, Journal Name: Review of Scientific Instrumentation Journal Issue: 1 Vol. 76
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
DESIGN
EXCITED STATES
FLUORESCENCE
IONIZATION
PROBES
QUENCHING
RUTHENIUM
electrospray
instrumentation
stern-volmer