A study of the susceptibility of different mice strains to thio-TEPA: An experiment with recombinant strains

Journal Article · Sat Jul 01 04:00:00 UTC 1995 · Russian Journal of Genetics

OSTI ID:91051

Malashenko, A M; Beskova, T B ^[1]

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1. Scientific Research Laboratory for Experimental Biological Models, Moscow (Russian Federation)

The study is devoted to the clastogenic effect of thio-TEPA in raising recombinant strains 1XC3 in mice (obtained from 101/H x C3H/Sn crossing). By the summer of 1993, 15 strains had reached 10 generations of inbreeding. Five to six sibs of each strain (males and females) aged 6-8 weeks were treated with the mutagen (3 mg/kg, i/p). Strain sensitivity was estimated by the frequency of marrow cells exhibiting chromosome lesions (including gaps). According to this characteristic, the strains were divided into two unequal groups. The first one included 12 less-sensitive strains (chromosome aberrations were present, on average, in 33.5% of the cells, and lay in the range from 27.0{+-}4.5 to 39.8{+-}4.9). The second group included three more-sensitive strains with 51.2, 51.7, and 59.5% of the cells being affected (54.1% on average). A similar difference between the groups was found with respect to the number of breaks per cell: 1.15 in resistant strains and 2.88 in sensitive ones. This difference was significant and similar to that found earlier for C3H and 101 strains. Thus, the results obtained support the hypothesis that mice of the 101/H strain carry a gene enhancing sensitivity to chemical mutagens (mut-l). Some strains of the first group were eliminated; the remaining ones will be bred until the 20th generation, when they will be investigated again in more detail. 24 refs., 1 fig., 2 tabs.

• OSTI ID: 91051
• Journal Information: Russian Journal of Genetics, Journal Name: Russian Journal of Genetics Journal Issue: 7 Vol. 31; ISSN 1022-7954; ISSN RJGEEQ
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
56 BIOLOGY AND MEDICINE
APPLIED STUDIES
ANIMAL BREEDING
BONE MARROW CELLS
CHROMOSOMAL ABERRATIONS
CHROMOSOMES
GENE MUTATIONS
GENES
GENETIC EFFECTS
MICE
MUTAGENS