Bacterial inosine 5'-monophosphate dehydrogenase ("IMPDH") DNA as a dominant selectable marker in mammals and other eukaryotes
Patent
·
OSTI ID:902644
- Chicago, IL
- Woodridge, IL
The present invention relates to a nucleic acid sequence and its corresponding protein sequence useful as a dominant selectable marker in eukaryotes. More specifically the invention relates to a nucleic acid encoding a bacterial IMPDH gene that has been engineered into a eukaryotic expression vectors, thereby permitting bacterial IMPDH expression in mammalian cells. Bacterial IMPDH expression confers resistance to MPA which can be used as dominant selectable marker in eukaryotes including mammals. The invention also relates to expression vectors and cells that express the bacterial IMPDH gene as well as gene therapies and protein synthesis.
- Research Organization:
- Argonne National Laboratory (ANL), Argonne, IL; University of Chicago
- Sponsoring Organization:
- United States Department of Energy
- DOE Contract Number:
- W-31109-ENG-38
- Assignee:
- The United States of America as represented by the United States Department of Energy (Washington, DC)
- Patent Number(s):
- 7,183,106
- Application Number:
- 10/856,409
- OSTI ID:
- 902644
- Country of Publication:
- United States
- Language:
- English
Bacterial IMPDH Gene Used for the Selection of Mammalian Cell Transfectants
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journal | June 2003 |
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