Methods and materials relating to IMPDH and GMP production

Collart, Frank R; Huberman, Eliezer

Methods and materials relating to IMPDH and GMP production

Patent · Wed Jan 01 04:00:00 EST 1997

OSTI ID:871138

Collart, Frank R ^[1]; Huberman, Eliezer ^[2]

1056 Crestwood La., Bolingbrook, IL 60439
424 Sunset Ave., LaGrange, IL 60525

Disclosed are purified and isolated DNA sequences encoding eukaryotic proteins possessing biological properties of inosine 5'-monophosphate dehydrogenase ("IMPDH"). Illustratively, mammalian (e.g., human) IMPDH-encoding DNA sequences are useful in transformation or transfection of host cells for the large scale recombinant production of the enzymatically active expression products and/or products (e.g., GMP) resulting from IMPDH catalyzed synthesis in cells. Vectors including IMPDH-encoding DNA sequences are useful in gene amplification procedures. Recombinant proteins and synthetic peptides provided by the invention are useful as immunological reagents and in the preparation of antibodies (including polyclonal and monoclonal antibodies) for quantitative detection of IMPDH.

View Patent

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL

Assignee:: Collart, Frank R. (1056 Crestwood La., Bolingbrook, IL 60439);Huberman, Eliezer (424 Sunset Ave., LaGrange, IL 60525)

Patent Number(s):: US 5665583

OSTI ID:: 871138

Country of Publication:: United States

Language:: English

References (39)

A general immunochemical method for detecting proteins on blots Kittler, Jason M.; Meisler, Natalie T.; Viceps-Madore, Dace Analytical Biochemistry, Vol. 137, Issue 1 https://doi.org/10.1016/0003-2697(84)90372-5	journal	February 1984
The inhibition of nucleic acid synthesis by mycophenolic acid Franklin, T. J.; Cook, Jennifer M. Biochemical Journal, Vol. 113, Issue 3 https://doi.org/10.1042/bj1130515	journal	July 1969
Metallothionein-human GH fusion genes stimulate growth of mice Palmiter, R.; Norstedt, G.; Gelinas, R. Science, Vol. 222, Issue 4625 https://doi.org/10.1126/science.6356363	journal	November 1983
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Towbin, H.; Staehelin, T.; Gordon, J. Proceedings of the National Academy of Sciences, Vol. 76, Issue 9, p. 4350-4354 https://doi.org/10.1073/pnas.76.9.4350	journal	September 1979
Action of the active metabolites of tiazofurin and ribavirin on purified IMP dehydrogenase Yamada, Yasukazu; Natsumeda, Yutaka; Weber, George Biochemistry, Vol. 27, Issue 6 https://doi.org/10.1021/bi00406a057	journal	March 1988
[2] Production of antisera with small doses of immunogen: multiple intradermal injections Vaitukaitis, Judith L. Methods in Enzymology https://doi.org/10.1016/0076-6879(81)73055-6	book	January 1981
Differential expression of three alpha-tubulin genes in Chinese hamster ovary cells Elliott, E. M.; Okayama, H.; Sarangi, F. Molecular and Cellular Biology, Vol. 5, Issue 1 https://doi.org/10.1128/mcb.5.1.236-241.1985	journal	January 1985
Cloning of the Bacillus Subtilis IMP Dehydrogenase Gene and its Application to Increased Production of Guanosine Miyagawa, Ken-ichiro; Kimura, Hiroyuki; Nakahama, Kazuo Nature Biotechnology, Vol. 4, Issue 3 https://doi.org/10.1038/nbt0386-225	journal	March 1986
A comprehensive set of sequence analysis programs for the VAX Devereux, John; Haeberli, Paul; Smithies, Oliver Nucleic Acids Research, Vol. 12, Issue 1, p. 387-395 https://doi.org/10.1093/nar/12.1Part1.387	journal	January 1984
Amplification of the IMP dehydrogenase gene in Chinese hamster cells resistant to mycophenolic acid. Collart, F. R.; Huberman, E. Molecular and Cellular Biology, Vol. 7, Issue 9 https://doi.org/10.1128/MCB.7.9.3328	journal	September 1987
High-efficiency cloning of full-length cDNA Okayama, H.; Berg, P. Molecular and Cellular Biology, Vol. 2, Issue 2 https://doi.org/10.1128/mcb.2.2.161-170.1982	journal	February 1982
Nucleotide sequence of the guaB locus encoding IMP dehydrogenaseof Escherichia coli K12 Tiedeman, Amelia A.; Smith, John M. Nucleic Acids Research, Vol. 13, Issue 4 https://doi.org/10.1093/nar/13.4.1303	journal	January 1985
A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity Feinberg, Andrew P.; Vogelstein, Bert Analytical Biochemistry, Vol. 132, Issue 1 https://doi.org/10.1016/0003-2697(83)90418-9	journal	July 1983
Introduction of Genetic Material into Plant Cells Caplan, A.; Herrera-Estrella, L.; Inze, D. Science, Vol. 222, Issue 4625 https://doi.org/10.1126/science.222.4625.815	journal	November 1983
Degranulation and abnormal bactericidal function of granulocytes procured by reversible adhesion to nylon wool Klock, Jc; Bainton, Df Blood, Vol. 48, Issue 1 https://doi.org/10.1182/blood.V48.1.149.bloodjournal481149	journal	July 1976
Isolation of a human anti-haemophilic factor IX cDNA clone using a unique 52-base synthetic oligonucleotide probe deduced from the amino acid sequence of bovine factor IX Jaye, Michael; Salle, Henri de la; Schamber, Fabienne Nucleic Acids Research, Vol. 11, Issue 8 https://doi.org/10.1093/nar/11.8.2325	journal	January 1983
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease Chirgwin, John M.; Przybyla, Alan E.; MacDonald, Raymond J. Biochemistry, Vol. 18, Issue 24 https://doi.org/10.1021/bi00591a005	journal	November 1979
Purification of IMP dehydrogenase from rat hepatoma 3924A Ikegami, Tadashi; Natsumeda, Yutaka; Weber, George Life Sciences, Vol. 40, Issue 23 https://doi.org/10.1016/0024-3205(87)90064-6	journal	June 1987
Biochemical commitment to replication in cancer cells Weber, George; Olah, Edith; Lui, May S. Advances in Enzyme Regulation, Vol. 18 https://doi.org/10.1016/0065-2571(80)90005-9	journal	January 1980
Introduction of Novel Genes into Fish Maclean, N.; Penman, D.; Zhu, Z. Bio/Technology, Vol. 5, Issue 3 https://doi.org/10.1038/nbt0387-257	journal	March 1987
Coordinated increase in activities of the signal transduction enzymes PI kinase and PIP kinase in human cancer cells Singhal, Radhey L.; Albert Yeh, Y.; Look, Katherine Y. Life Sciences, Vol. 55, Issue 19 https://doi.org/10.1016/0024-3205(94)00690-3	journal	January 1994
Guanine nucleotide depletion and toxicity in mouse T lymphoma (S-49) cells. Cohen, M. B.; Maybaum, J.; Sadee, W. Journal of Biological Chemistry, Vol. 256, Issue 16 https://doi.org/10.1016/S0021-9258(19)68902-7	journal	August 1981
DNA sequencing with chain-terminating inhibitors Sanger, F.; Nicklen, S.; Coulson, A. R. Proceedings of the National Academy of Sciences, Vol. 74, Issue 12 https://doi.org/10.1073/pnas.74.12.5463	journal	December 1977
A straightforward radiometric technique for measuring IMP dehydrogenase Cooney, David A.; Wilson, Yvonne; McGee, Ellen Analytical Biochemistry, Vol. 130, Issue 2 https://doi.org/10.1016/0003-2697(83)90597-3	journal	April 1983
Rapid electroelution of nucleic acids from agarose and acrylamide gels Zassenhaus, H. Peter; Butow, Ronald A.; Hannon, Yolanda P. Analytical Biochemistry, Vol. 125, Issue 1 https://doi.org/10.1016/0003-2697(82)90392-X	journal	September 1982
Purification and properties of inosine monophosphate oxidoreductase from nitrogen-fixing nodules of cowpea (Vigna unguiculata L. Walp) Atkins, C. A.; Shelp, B. J.; Storer, P. J. Archives of Biochemistry and Biophysics, Vol. 236, Issue 2 https://doi.org/10.1016/0003-9861(85)90687-3	journal	February 1985
IMP dehydrogenase, an enzyme linked with proliferation and malignancy Jackson, Robert C.; Weber, George; Morris, Harold P. Nature, Vol. 256, Issue 5515 https://doi.org/10.1038/256331a0	journal	July 1975
IMP Dehydrogenase. II. Purification and Properties of the Enzyme from Yoshida Sarcoma Ascites Tumor Cells Okada, Masado; Shimura, Kentaro; Shiraki, Hiroshi The Journal of Biochemistry https://doi.org/10.1093/oxfordjournals.jbchem.a134508	journal	November 1983
Partial purification, properties and regulation of inosine 5′-phosphate dehydrogenase in normal and malignant rat tissues Jackson, R. C.; Morris, H. P.; Weber, G. Biochemical Journal, Vol. 166, Issue 1 https://doi.org/10.1042/bj1660001	journal	July 1977
Inosinic acid 5′-monophosphate dehydrogenase of Escherichia coli: Purification by affinity chromatography and some properties Krishnaiah, K. V. Archives of Biochemistry and Biophysics, Vol. 170 https://doi.org/10.1016/0003-9861(75)90152-6	journal	January 1975
A cDNA cloning vector that permits expression of cDNA inserts in mammalian cells. Okayama, H.; Berg, P. Molecular and Cellular Biology, Vol. 3, Issue 2 https://doi.org/10.1128/MCB.3.2.280	journal	February 1983
Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs Kozak, Marilyn Nucleic Acids Research, Vol. 12, Issue 2 https://doi.org/10.1093/nar/12.2.857	journal	January 1984
Album Saint Thomas More Lemasson, Béatrice Moreana, Vol. 20 (Number 78), Issue 2 https://doi.org/10.3366/more.1983.20.2.34	journal	June 1983
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4 Laemmli, U. K. Nature, Vol. 227, Issue 5259, p. 680-685 https://doi.org/10.1038/227680a0	journal	August 1970
Inosine 5′-monophosphate dehydrogenase of Escherichia coli. Purification by affinity chromatography, subunit structure and inhibition by guanosine 5′-monophosphate Gilbert, H. J.; Lowe, C. R.; Drabble, W. T. Biochemical Journal, Vol. 183, Issue 3 https://doi.org/10.1042/bj1830481	journal	December 1979
Mutagen-induced resistance to mycophenolic acid in hamster cells can be associated with increased inosine 5'-phosphate dehydrogenase activity. Huberman, E.; McKeown, C. K.; Friedman, J. Proceedings of the National Academy of Sciences, Vol. 78, Issue 5 https://doi.org/10.1073/pnas.78.5.3151	journal	May 1981
Calcium Phosphate Transfection of Nonadherent and Adherent Cells with Purified Plasmids Davis, Leonard G.; Dibner, Mark D.; Battey, James F. Basic Methods in Molecular Biology https://doi.org/10.1016/B978-0-444-01082-7.50076-X	book	January 1986
Characterization of mutant murine lymphoma cells with altered inosinate dehydrogenase activities. Ullman, B. Journal of Biological Chemistry, Vol. 258, Issue 1 https://doi.org/10.1016/S0021-9258(18)33286-1	journal	January 1983
rolA locus of the Ri plasmid directs developmental abnormalities in transgenic tobacco plants. Sinkar, V. P.; Pythoud, F.; White, F. F. Genes & Development, Vol. 2, Issue 6 https://doi.org/10.1101/gad.2.6.688	journal	June 1988

Similar Records

Bacterial IMPDH gene used for the selection of mammalian cell transfectants.

Journal Article · Sun Jun 01 00:00:00 EDT 2003 · BioTechiques · OSTI ID:961223

Bacterial inosine 5'-monophosphate dehydrogenase ("IMPDH") DNA as a dominant selectable marker in mammals and other eukaryotes

Patent · Mon Feb 26 23:00:00 EST 2007 · OSTI ID:902644

CTPS and IMPDH form cytoophidia in developmental thymocytes

Journal Article · Sun Aug 15 00:00:00 EDT 2021 · Experimental Cell Research · OSTI ID:23195597

Related Subjects

-monophosphate
/435/999/
active
amplification
amplification procedure
antibodies
biological
catalyzed
cells
dehydrogenase
detection
disclosed
dna
dna sequence
dna sequences
encoding
enzymatically
eukaryotic
expression
gmp
host
host cell
host cells
human
illustratively
immunological
impdh
impdh-encoding
including
inosine
isolated
isolated dna
mammalian
materials
methods
monoclonal
monoclonal antibodies
peptides
polyclonal
possessing
preparation
procedures
production
products
properties
proteins
provided
purified
quantitative
reagents
recombinant
recombinant production
recombinant protein
recombinant proteins
relating
resulting
scale
sequences
sequences encoding
synthesis
synthetic
transfection
transformation
useful
vectors

Methods and materials relating to IMPDH and GMP production

Citation Formats

References (39)

Similar Records

Related Subjects