Versatile Apoferritin Nanoparticle Labels for Assay of Protein

Journal Article · Mon Oct 30 04:00:00 EST 2006 · Analytical Chemistry, 78(21):7417-7423

DOI:https://doi.org/10.1021/ac060653j· OSTI ID:894838

Liu, Guodong; Wang, Jun; Wu, Hong; Lin, Yuehe

A versatile bioassay label based on marker-loaded-apoferritin nanoparticles (MLAN) have been developed for sensitive protein detection. Dissociation and reconstitution characteristics at different pH as well as the special cavity structure of apoferritin provide a facile route to prepare nanoparticle labels, and avoid complicated and tedious synthesis process of conventional nanoparticle labels. The optical and electrochemical characteristics of the prepared nanoparticle labels are easily controlled by loading different optical or electrochemical markers. A fluorescence marker (fluorescein anion) and a redox marker [hexacyanoferrate (III)] were used as model markers to load into the cavity of apoferritin nanoparticle and developed for microscopic fluorescence immunoassay and electrochemical immunoassay, respectively. Detection limits of 0.06 ng mL-1 (0.39 pM) and 0.08 ng mL-1 (0.51 pM) of IgG were obtained with fluorescein MLAP and hexacyanoferrate MLAN, respectively. The new nanoparticle labels hold great promise for multiplex protein detection (in connection to nanoparticles loaded with different markers) and for enhancing the sensitivity of other bioassay.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 894838
• Report Number(s): PNNL-SA-50120; 16739; 17505
• Journal Information: Analytical Chemistry, 78(21):7417-7423, Journal Name: Analytical Chemistry, 78(21):7417-7423
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOASSAY
DETECTION
DISSOCIATION
Environmental Molecular Sciences Laboratory
FLUORESCEIN
FLUORESCENCE
IMMUNOASSAY
PROTEINS
SENSITIVITY
SYNTHESIS
biomarker
nanoparticle
proten