Bioassay Labels Based on Apoferritin Nanovehicles

Journal Article · Mon Sep 04 04:00:00 EDT 2006 · Chembiochem, 7(9):1315-1319

DOI:https://doi.org/10.1002/cbic.200600225· OSTI ID:891125

Liu, Guodong; Wang, Jun; Lea, Alan S; Lin, Yuehe

Here we report a nanoparticle label based on apoferritin nanovehicle loaded internally with markers for sensitive electrochemical DNA detection. The central cavity structure, the dissociation and reconstitute properties at different pHs of apoferritin provide a facile method to load and release markers. Hexacynoferrate(III) was used as model marker to load into the cavity of apoferritin protein cage. The loaded nanoparticle surface was functionalized with amino-modified DNA probe. Electrochemical DNA hybridization assay based on the hexacynoferrate loaded apoferritin nanovehicle could detect 23 atmol DNA targets in 50 ul sample solution. The concept could be readily extended to load other redox and fluorescence markers for bioassay applications. The new nanoparticle labels hold great promise for multi-target detection (in connection to nanoparticles loaded with different markers) and for enhancing the sensitivity of other bioassays.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• DOE Contract Number: AC05-76RL01830
• OSTI ID: 891125
• Report Number(s): PNNL-SA-47959; 12490
• Journal Information: Chembiochem, 7(9):1315-1319, Journal Name: Chembiochem, 7(9):1315-1319
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BIOASSAY
DETECTION
DISSOCIATION
DNA
DNA HYBRIDIZATION
Environmental Molecular Sciences Laboratory
FLUORESCENCE
PROTEINS
SENSITIVITY
TARGETS
bioassay
nanoparticle