Dosage-Dependent Proteome Response of Shewanella oneidensis MR-1 to Chromate Insult

Conference · Wed Apr 05 04:00:00 EDT 2006

OSTI ID:894627

Thompson, Melissa R; VerBerkmoes, Nathan C; Chourey, Karuna; Brown, Steven D; Hettich, Robert L; Thompson, Dorothea K

Shewanella oneidensis MR-1 is a gram-negative, facultatively anaerobic bacterium originally isolated from a freshwater lake. S. oneidensis MR-1 has the ability to reduce toxic metal ions [e.g., Cr(VI) and U(VI)] found in industrial and governmental waste sites. Cells were grown and exposed to three different metal concentrations in order to probe the dosage response of S. oneidensis MR-1 to Cr(VI) in the form of chromate. Protein fractions were digested with trypsin and analyzed with a multidimensional HPLC-NanoESIMS/MS protocol. The goal of this work is to identify protein components of pathways/mechanisms responsible for both detoxification and reduction of chromate.

• Research Organization: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN; Purdue University, West Lafayette, IN
• Sponsoring Organization: USDOE - Office of Science (SC)
• DOE Contract Number: AC05-00OR22725
• OSTI ID: 894627
• Report Number(s): CONF-ERSP2006-44
• Country of Publication: United States
• Language: English

Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
CHROMATES
DETOXIFICATION
PROBES
PROTEINS
TRYPSIN
WASTES