The accumulation of stable cytogenetic rearrangements with age-determined by chromosome painting

Journal Article · Sat Dec 31 04:00:00 EST 1994 · Environmental and Molecular Mutagenesis

OSTI ID:88910

Ramsey, M J; Lee, D A; Senft, J R; Briner, J F; Moore, II, D H; Tucker, J D ^[1]

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1. Lawrence Livermore National Laboratory, CA (United States)

Chromosome painting is a rapid method of quantifying structural chromosomal rearrangements. The method is particularly useful for detecting stable aberrations which are difficult and expensive to quantify with classical methods. Translocations, being inherently stable, can be used as a biodosimeter for chronic and temporally-displaced exposure to radiation. Translocations may also be useful for quantifying chronic exposure to environmentally related agents which may result in an accumulation of cytogenetic damage with age. Because most chemical exposures are low and chronic, conventional cytogenetic methods are not expected to be informative. To understand the extent that age and lifestyle factors impact the frequency of stable aberrations, we used chromosome painting in healthy individuals who have not been occupationally or accidentally exposed to radiation or chemicals, and who have not received chemo- or radiotherapy. To date we have analyzed 15 umbilical cord bloods as well as peripheral blood samples from 83 adults aged up to 77 years. Because stable aberrations are rare in unexposed people, we have scored large numbers of cells from each subject. Thus far we have analyzed the equivalent of more than 78,000 metaphases from these 83 people, and have observed an average of 0.75% of cells with translocations or stable insertions. A significant curvilinear relationship with age is apparent (R{sup 2} = 0.69, p <0.00001). No effect with smoking was seen.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 88910
• Report Number(s): CONF-9405324--
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.23 Vol. 23; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
AGE DEPENDENCE
CHROMOSOMAL ABERRATIONS
EVALUATION
HUMAN CHROMOSOMES