Cytogenetic comparison of three control populations as measured by chromosome painting

Journal Article · Wed Oct 01 04:00:00 EDT 1997 · Environmental and Molecular Mutagenesis

OSTI ID:530858

Ramsey, M J; Nelson, D O ^[1]; Long-Simpson, L ^[2]

---

1. Lawrence Livermore National Lab., CA (United States)
2. Univ. of Minnesota, Minneapolis, MN (United States); and others

To quantify the effects of adverse exposure, it is important that the baseline frequency of stable aberrations be well established. It is also important to understand how control populations in different demographic areas vary in their aberration frequencies. Recently we used chromosome painting to show that translocations accumulate with age and cigarette smoking. Here we compare three control populations, one each from Minnesota, California, and Russia, for aberration frequencies as measured by whole chromosome painting of chromosomes 1, 2 and 4. All subjects were healthy individuals who had not been occupationally or accidentally exposed to radiation or chemicals, and who had not received chemo- or radio-therapy. Data from the three populations were combined and fit to a series of models that regressed a transformed value of stable translocation on sample origin, age, and smoking status. While there was some evidence (p = 0.025), with an approximately 30% difference in translocation frequencies between the California and Minnesota samples (p = 0.015). The difference between the American populations may be explained by the experimental protocols used. In the California (and Russian) populations, the cells were cultured for 52f hours, but in the Minnesota population study the cells were grown for 72 hours. Whether this difference is responsible for the variation in translocation frequencies is unknown, but should be explored further.

• DOE Contract Number: W-7405-ENG-48
• OSTI ID: 530858
• Report Number(s): CONF-9704100--
• Journal Information: Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.28 Vol. 29; ISSN 0893-6692; ISSN EMMUEG
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMAL ABERRATIONS
COMPARATIVE EVALUATIONS
HUMAN POPULATIONS