Induction of micronuclei in mouse keratinocytes by polycyclic aromatic hydrocarbons and n-heterocyclic aromatics
Journal Article
·
· Environmental and Molecular Mutagenesis
OSTI ID:88890
- Univ. of Cincinnati, OH (United States)
This research is concerned with the ability of environmental carcinogens, benzo[a]pyrene (BaP), 7,12-dimethylbenz[a]anthracene (DMBA) and 7H-dibenzo(c,g)carbazole (DBC) to induce micronuclei (MN) in mouse keratinocytes. Mouse (HSD:ICR[Br]) keratinocytes (primary cultures) are grown on collagen coated microscope slides/dishes and dosed with the carcinogen (dissolved in 0.5% DMSO) for 12/24 hours. After carcinogen treatment, the medium is replaced with the medium containing cytochalasin-B and the cells are cultures for 72 hours. The binucleate fraction is used as a general indicator of cytotoxicity. MN are scored in 1000 acridine organs stained binucleated cells. Adriamycin, a direct acting compound, is used as a positive control. DBC induces a dose-dependent (0.01-1 {mu}g/ml) increase in MN (13-40.3 MN/1000 BN cells ) over controls (9.7{+-}1.8); N=3). In addition, 1 nmole/ml (256 ng/ml of DMBA induces twice the number of MN over controls (21.5{+-}1.1). In contrast, skin painting of higher non-cytotoxic doses of BaP (12 {mu}g/ml; 24 {mu}g/ml) showed BaP to be a weak inducer of MN over controls. Similar results were obtained in vitro. The lack of a significant response with BaP indicates that negative results in the mouse kerotinocyte MN assay should be interpreted with caution.
- OSTI ID:
- 88890
- Report Number(s):
- CONF-9405324--; CNN: Grant 5 R03 OH02880-02
- Journal Information:
- Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.23 Vol. 23; ISSN 0893-6692; ISSN EMMUEG
- Country of Publication:
- United States
- Language:
- English
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