Influence of organomercurials on the induction chromosome alterations in CHO cells
Journal Article
·
· Environmental and Molecular Mutagenesis
OSTI ID:88888
- Kentucky State Univ., Frankfort, KY (United States)
Mercury is well known as a toxic heavy metal and protoplasmic poison. In order to further assess its effect on cell`s genetic material, the influence of organomercurials, phenylmercuric chloride and ethylmercuric chloride on chromosome aberrations (CA) and sister chromatid exchanges (SCE) on Chinese hamster ovary (CHO) cells was investigated. The cell cultures were treated with 10{sup -8}, 10{sup -7}, 10{sup -6}, and 10{sup -5} of phenyl and ethylmercuric chloride. It was observed that higher concentrations (10{sup -6} and 10{sup -5}M) of phenylmercuric chloride substantially elevated the frequency of CA compared to the controls. The increase appeared to be dose dependent. In 10{sup -5} M level, phenylmercuric chloride produced excessive endoreduplication of chromosomes. The compound also raised the rates of SCE but the effect did not appear to be dose dependent. Compared to this, ethylmercuric chloride was less effective in increasing the rates of above parameters compared to the controls. The effect produced by organomercurials may be related to the binding sites in the cell. Mercury compounds are known to interact with the cell`s spindle apparatus because of their affinity for thiol groups.
- OSTI ID:
- 88888
- Report Number(s):
- CONF-9405324--; CNN: Grant S14 GM 05231
- Journal Information:
- Environmental and Molecular Mutagenesis, Journal Name: Environmental and Molecular Mutagenesis Journal Issue: Suppl.23 Vol. 23; ISSN 0893-6692; ISSN EMMUEG
- Country of Publication:
- United States
- Language:
- English
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